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The extract was also investigated for its antiproliferative activity. The methanol extract showed a significant antiproliferative effect against L-929 and HTC-t cells with IC50 values of 360 and 546.8 g/mL respectively. These results suggest that the extract is a potential source of antiproliferative substances.721

The extract was also screened in an MTT cytotoxicity assay to assess its cytotoxicity against CHL-1, HTC-t, and L-929 cell lines. The extract was non-toxic to CHL-1 and L-929 cells with IC50 values of 1.1 and 7.8 mg/mL respectively, but was toxic to HTC-t cells with an IC50 value of 2.7 mg/mL.729

The total phenolic and flavonoid contents of the extract were measured, and hesperidin was identified as a major polyphenol present in the extract and at concentrations up to 2.5%, inhibiting the formation of hydroxyl free radicals. Hesperidin is an antioxidant and a potential cancer chemopreventive agent, but there are very few publications concerning hesperidin in natural products..20.37

The present study suggests that hesperidin, a polyphenol frequently observed in citrus fruits and red-fleshed radishes, may be a major phytochemical responsible for the antioxidant activities of HHT, and that hesperidin may be a potential new source of natural antioxidants for food use.

The molecular docking studies indicate that hesperetin can bind firmly to the active site of COX-1, competing with the substrate for the enzyme. It is not yet established if this is a general mechanism for the inhibition of COX-1 by flavonoids, but the results indicated a good fit between the poses and the ligand-binding site.56.

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